› 3op1

X-ray diffraction
2.49Å resolution

Crystal Structure of Macrolide-efflux Protein SP_1110 from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb3op1/pdb
PDB entry 3op1 coloured by chain, front view.
PDB entry 3op1 coloured by chain, side view.
PDB entry 3op1 coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Entry authors: Kim Y, Li H, Cobb G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reactions catalysed: 
ATP + riboflavin = ADP + FMN
ATP + FMN = diphosphate + FAD
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101439 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin biosynthesis protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 308 amino acids
Theoretical weight: 35.21 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A0H2UPY5 (Residues: 1-305; Coverage: 100%)
Gene name: SP_1110
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SO4 4 x SO4
Ligand GOL 12 x GOL
Ligand PEG 4 x PEG
Ligand ACY 1 x ACY
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 180.153Å b: 73.247Å c: 87.518Å
α: 90° β: 112.48° γ: 90°
R-values:
R R work R free
0.202 0.198 0.265
Expression system: Escherichia coli BL21

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