› 3on4

X-ray diffraction
1.85Å resolution

Crystal structure of TetR transcriptional regulator from Legionella pneumophila

Released:
DOI: 10.2210/pdb3on4/pdb
PDB entry 3on4 coloured by chain, front view.
PDB entry 3on4 coloured by chain, side view.
PDB entry 3on4 coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Entry authors: Michalska K, Li H, Gu M, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 191 amino acids
Theoretical weight: 21.41 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5ZU41 (Residues: 1-188; Coverage: 100%)
Gene name: lpg1967
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 81.38Å b: 124.99Å c: 151.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.218
Expression system: Escherichia coli BL21

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