› 3ocx

X-ray diffraction
1.9Å resolution

Structure of Recombinant Haemophilus influenzae e(P4) Acid Phosphatase mutant D66N complexed with 2'-AMP

Released:
DOI: 10.2210/pdb3ocx/pdb
PDB entry 3ocx coloured by chain, front view.
PDB entry 3ocx coloured by chain, side view.
PDB entry 3ocx coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Recognition of nucleoside monophosphate substrates by Haemophilus influenzae class C acid phosphatase.
Singh H, Schuermann JP, Reilly TJ, Calcutt MJ, Tanner JJ
J Mol Biol 404 639-49 (2010)
PMID: 20934434

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipoprotein E Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 29.62 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26093 (Residues: 22-274; Coverage: 100%)
Gene names: HI_0693, hel, ompP4
Sequence domains: HAD superfamily, subfamily IIIB (Acid phosphatase)
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

2 bound ligands:
Ligand 2AM 1 x 2AM
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P6522
Unit cell:
a: 97.87Å b: 97.87Å c: 107.598Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 citation in other articles

The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism.
Garavaglia et al. (2012)
9 more

2 mentions without citation

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria.
Vaca et al. (2020)
1 more