PDBe 3o7l

X-ray diffraction
2.8Å resolution

Crystal Structure of phospholamban (1-19):PKA C-subunit:AMP-PNP:Mg2+ complex

Released:
Source organism: Mus musculus

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cardiac phospholamban Chain: I
Molecule details ›
Chain: I
Length: 15 amino acids
Theoretical weight: 1.74 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P61014 (Residues: 4-18; Coverage: 29%)
Gene name: Pln
cAMP-dependent protein kinase catalytic subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 350 amino acids
Theoretical weight: 40.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase catalytic subunit alpha Chain: D
Molecule details ›
Chain: D
Length: 350 amino acids
Theoretical weight: 40.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P61
Unit cell:
a: 92.193Å b: 92.193Å c: 192.186Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.216 0.287
Expression systems:
  • Not provided
  • Escherichia coli