› 3o4n

X-ray diffraction
1.8Å resolution

Crystal structure of the Rous Associated Virus Integrase catalytic domain in MES buffer pH 6.0

Released:
DOI: 10.2210/pdb3o4n/pdb
PDB entry 3o4n coloured by chain, front view.
PDB entry 3o4n coloured by chain, side view.
PDB entry 3o4n coloured by chain, top view.
Source organism: Rous sarcoma virus
Primary publication:
A crystal structure of the catalytic core domain of an avian sarcoma and leukemia virus integrase suggests an alternate dimeric assembly.
Ballandras A, Moreau K, Robert X, Confort MP, Merceron R, Haser R, Ronfort C, Gouet P
PLoS One 6 e23032 (2011)
PMID: 21857987

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.33 KDa
Source organism: Rous sarcoma virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O92956 (Residues: 1333-1479; Coverage: 9%)
Gene name: gag-pol
Sequence domains: Integrase core domain
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

2 bound ligands:
Ligand MES 1 x MES
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P61
Unit cell:
a: 107.5Å b: 107.5Å c: 50.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.189 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Advances on Innate Immune Evasion by Avian Immunosuppressive Viruses.
Wang et al. (2022)
4 other articles

1 mention without citation

A crystal structure of the catalytic core domain of an avian sarcoma and leukemia virus integrase suggests an alternate dimeric assembly.
Ballandras et al. (2011)