› 3o1l

X-ray diffraction
2.2Å resolution

Crystal structure of a formyltetrahydrofolate deformylase (PSPTO_4314) from Pseudomonas syringae pv. tomato str. DC3000 at 2.20 A resolution

Released:
DOI: 10.2210/pdb3o1l/pdb
PDB entry 3o1l coloured by chain, front view.
PDB entry 3o1l coloured by chain, side view.
PDB entry 3o1l coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-183395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formyltetrahydrofolate deformylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 35.32 KDa
Source organism: Pseudomonas syringae pv. tomato
Expression system: Escherichia coli
UniProt:
  • Canonical: Q87X74 (Residues: 1-283; Coverage: 100%)
Gene names: PSPTO_4314, purU, purU-3
Sequence domains: Formyl transferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 8 x GOL
Ligand SO4 9 x SO4
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P41212
Unit cell:
a: 131.271Å b: 131.271Å c: 102.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.202
Expression system: Escherichia coli

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