› 3nxc

X-ray diffraction
2.5Å resolution

Molecular mechanism by which the Escherichia coli nucleoid occlusion factor, SlmA, keeps cytokinesis in check

Released:
DOI: 10.2210/pdb3nxc/pdb
PDB entry 3nxc coloured by chain, front view.
PDB entry 3nxc coloured by chain, side view.
PDB entry 3nxc coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check.
Tonthat NK, Arold ST, Pickering BF, Van Dyke MW, Liang S, Lu Y, Beuria TK, Margolin W, Schumacher MA
EMBO J 30 154-64 (2011)
PMID: 21113127

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoid occlusion factor SlmA Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 24.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C093 (Residues: 1-198; Coverage: 100%)
Gene names: JW5641, b3641, slmA, ttk, yicB
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3221
Unit cell:
a: 50.38Å b: 50.38Å c: 121.32Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.224 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

The physiology of bacterial cell division.
Egan et al. (2013)
25 more

2 mentions without citation

Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check.
Tonthat et al. (2011)
1 more