PDBe 3nvm

X-ray diffraction
3.41Å resolution

Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nop domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 371 amino acids
Theoretical weight: 42.95 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4M1 (Residues: 1-369; Coverage: 90%)
Gene name: PF0060
Sequence domains: snoRNA binding domain, fibrillarin
Structure domains:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase Chain: B
Molecule details ›
Chain: B
Length: 234 amino acids
Theoretical weight: 26.76 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U4M2 (Residues: 1-227; Coverage: 100%)
Gene names: PF0059, flpA
Sequence domains: Fibrillarin
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3112
Unit cell:
a: 100.623Å b: 100.623Å c: 265.355Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.242 0.316
Expression system: Escherichia coli