› 3nuq

X-ray diffraction
1.7Å resolution

Structure of a putative nucleotide phosphatase from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3nuq/pdb
PDB entry 3nuq coloured by chain, front view.
PDB entry 3nuq coloured by chain, side view.
PDB entry 3nuq coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry authors: Dong A, Yang C, Singer AU, Evdokimova E, Kudritsdka M, Brown G, Edwards AM, Joachimiak A, Savchenko A, Yakunin AF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156712 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Suppressor of disruption of TFIIS Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53078 (Residues: 1-280; Coverage: 100%)
Gene names: SDT1, SSM1, YGL224C
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand EDO 2 x EDO
Ligand NA 1 x NA
Ligand CL 2 x CL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E DW
Spacegroup: P212121
Unit cell:
a: 58.474Å b: 64.664Å c: 67.708Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.226
Expression system: Escherichia coli BL21(DE3)

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