› 3nuk

X-ray diffraction
2.06Å resolution

THE CRYSTAL STRUCTURE OF THE W169Y mutant of ALPHA-GLUCOSIDASE (FAMILY 31) from RUMINOCOCCUS OBEUM ATCC 29174

Released:
DOI: 10.2210/pdb3nuk/pdb
PDB entry 3nuk coloured by chain, front view.
PDB entry 3nuk coloured by chain, side view.
PDB entry 3nuk coloured by chain, top view.
Source organism: Blautia obeum ATCC 29174
Entry authors: Tan K, Tesar C, Wilton R, Keigher L, Babnigg G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase, family 31 Chains: A, B
Molecule details ›
Chains: A, B
Length: 666 amino acids
Theoretical weight: 77.43 KDa
Source organism: Blautia obeum ATCC 29174
Expression system: Escherichia coli
UniProt:
  • Canonical: A5ZY13 (Residues: 1-663; Coverage: 100%)
Gene name: RUMOBE_03919
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 68.798Å b: 125.531Å c: 88.296Å
α: 90° β: 107.82° γ: 90°
R-values:
R R work R free
0.197 0.194 0.247
Expression system: Escherichia coli

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