› 3nsl

X-ray diffraction
1.5Å resolution

Crystal Structure of S100A3 C30A+C68A double mutant expressed in insect cell

Released:
DOI: 10.2210/pdb3nsl/pdb
PDB entry 3nsl coloured by chain, front view.
PDB entry 3nsl coloured by chain, side view.
PDB entry 3nsl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Refined crystal structures of human Ca(2+)/Zn(2+)-binding S100A3 protein characterized by two disulfide bridges.
Unno M, Kawasaki T, Takahara H, Heizmann CW, Kizawa K
J Mol Biol 408 477-90 (2011)
PMID: 21377473

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein S100-A3 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 101 amino acids
Theoretical weight: 11.56 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P33764 (Residues: 2-101; Coverage: 99%)
Gene names: S100A3, S100E
Sequence domains: S-100/ICaBP type calcium binding domain
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P31
Unit cell:
a: 101.357Å b: 101.357Å c: 45.209Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.197
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Building the niche: the role of the S100 proteins in metastatic growth.
Lukanidin et al. (2012)
3 more

3 mentions without citation

PRISMOID: a comprehensive 3D structure database for post-translational modifications and mutations with functional impact.
Li et al. (2020)
2 more