› 3nrz

X-ray diffraction
1.8Å resolution

Crystal Structure of Bovine Xanthine Oxidase in Complex with Hypoxanthine

Released:
DOI: 10.2210/pdb3nrz/pdb
PDB entry 3nrz coloured by chain, front view.
PDB entry 3nrz coloured by chain, side view.
PDB entry 3nrz coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Substrate orientation and catalytic specificity in the action of xanthine oxidase: the sequential hydroxylation of hypoxanthine to uric acid.
Cao H, Pauff JM, Hille R
J Biol Chem 285 28044-53 (2010)
PMID: 20615869

Function and Biology Details

Reactions catalysed: 
Xanthine + NAD(+) + H(2)O = urate + NADH
Xanthine + H(2)O + O(2) = urate + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160414 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Xanthine dehydrogenase/oxidase Chains: A, J
Molecule details ›
Chains: A, J
Length: 164 amino acids
Theoretical weight: 18 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 2-165; Coverage: 12%)
Gene name: XDH
Sequence domains:
Structure domains:
Xanthine dehydrogenase/oxidase Chains: B, K
Molecule details ›
Chains: B, K
Length: 305 amino acids
Theoretical weight: 34.02 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 224-528; Coverage: 23%)
Gene name: XDH
Sequence domains:
Structure domains:
Xanthine dehydrogenase/oxidase Chains: C, L
Molecule details ›
Chains: C, L
Length: 756 amino acids
Theoretical weight: 83.1 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 571-1326; Coverage: 57%)
Gene name: XDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD

Cofactor: Ligand MTE 2 x MTE
3 bound ligands:
Ligand FES 4 x FES
Ligand MOS 2 x MOS
Ligand HPA 2 x HPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 133.016Å b: 73.645Å c: 138.696Å
α: 90° β: 97.05° γ: 90°
R-values:
R R work R free
0.199 0.197 0.234

Quick links

Citations

13 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
12 more

18 mentions without citation

Substrate orientation and catalytic specificity in the action of xanthine oxidase: the sequential hydroxylation of hypoxanthine to uric acid.
Cao et al. (2010)
17 more