› 3nq7

X-ray diffraction
1.44Å resolution

Crystal structure of the mutant F71A of orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with inhibitor BMP

Released:
DOI: 10.2210/pdb3nq7/pdb
PDB entry 3nq7 coloured by chain, front view.
PDB entry 3nq7 coloured by chain, side view.
PDB entry 3nq7 coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Entry authors: Fedorov AA, Fedorov EV, Wood BM, Gerlt JA, Almo SC

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate = UMP + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotidine 5'-phosphate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 24.81 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O26232 (Residues: 1-228; Coverage: 100%)
Gene names: MTH_129, pyrF
Sequence domains: Orotidine 5'-phosphate decarboxylase / HUMPS family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand BMP 2 x BMP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 59.587Å b: 64.23Å c: 61.458Å
α: 90° β: 115.54° γ: 90°
R-values:
R R work R free
0.18 0.178 0.207
Expression system: Escherichia coli

Quick links