› 3nj0

X-ray diffraction
1.89Å resolution

X-ray crystal structure of the PYL2-pyrabactin A complex

Released:
DOI: 10.2210/pdb3nj0/pdb
PDB entry 3nj0 coloured by chain, front view.
PDB entry 3nj0 coloured by chain, side view.
PDB entry 3nj0 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for selective activation of ABA receptors.
Peterson FC, Burgie ES, Park SY, Jensen DR, Weiner JJ, Bingman CA, Chang CE, Cutler SR, Phillips GN, Volkman BF
Nat Struct Mol Biol 17 1109-13 (2010)
PMID: 20729860

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131334 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Abscisic acid receptor PYL2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 193 amino acids
Theoretical weight: 21.59 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O80992 (Residues: 1-190; Coverage: 100%)
Gene names: At2g26040, PYL2, RCAR14, T19L18.15
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

3 bound ligands:
Ligand PYV 3 x PYV
Ligand PEG 2 x PEG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2221
Unit cell:
a: 62.542Å b: 105.151Å c: 187.231Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.178 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

13 review citations

Ion channels in plants.
Hedrich R. (2012)
12 more

3 mentions without citation

Chemical Manipulation of Abscisic Acid Signaling: A New Approach to Abiotic and Biotic Stress Management in Agriculture.
Hewage et al. (2020)
2 more