PDBe 3nbn

X-ray diffraction
3.45Å resolution

Crystal structure of a dimer of Notch Transcription Complex trimers on HES1 DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Mastermind-like protein 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 63 amino acids
Theoretical weight: 7.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92585 (Residues: 13-74; Coverage: 6%)
Gene names: KIAA0200, MAML1
Sequence domains: MamL-1 domain
Notch 1 intracellular domain Chains: B, E
Molecule details ›
Chains: B, E
Length: 256 amino acids
Theoretical weight: 27.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1872-2126; Coverage: 10%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Recombining binding protein suppressor of hairless Chains: A, D
Molecule details ›
Chains: A, D
Length: 433 amino acids
Theoretical weight: 49.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06330 (Residues: 23-448; Coverage: 85%)
  • Best match: Q06330-5 (Residues: 1-413)
Gene names: IGKJRB, IGKJRB1, RBPJ, RBPJK, RBPSUH
Sequence domains:
Structure domains:
DNA, HES1 promoter Chain: X
Molecule details ›
Chain: X
Length: 37 nucleotides
Theoretical weight: 11.53 KDa
DNA, HES1 promoter Chain: Y
Molecule details ›
Chain: Y
Length: 37 nucleotides
Theoretical weight: 11.21 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 295.112Å b: 108.059Å c: 87.239Å
α: 90° β: 102.52° γ: 90°
R-values:
R R work R free
0.256 0.254 0.298
Expression system: Escherichia coli