3n7c

X-ray diffraction
2.26Å resolution

Crystal structure of the RAN binding domain from the nuclear pore complex component NUP2 from Ashbya gossypii

Released:
Source organism: Eremothecium gossypii
Entry authors: Sampathkumar P, Manglicmot D, Gilmore J, Bain K, Gheyi T, Atwell S, Thompson DA, Emtage JS, Wasserman S, Sauder JM, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-181110 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RanBD1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.82 KDa
Source organism: Eremothecium gossypii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q75DJ0 (Residues: 495-613; Coverage: 19%)
Gene name: AGOS_ABR034W
Sequence domains: RanBP1 domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 57.54Å b: 62.55Å c: 76.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.234 0.293
Expression system: Escherichia coli BL21(DE3)