PDBe 3n7b

X-ray diffraction
2.65Å resolution

SgrAI bound to secondary site DNA and Ca(II)

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
SgraIR restriction enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 37.94 KDa
Source organism: Streptomyces griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9F6L0 (Residues: 2-339; Coverage: 100%)
Gene name: sgraIR
Sequence domains: Cfr10I/Bse634I restriction endonuclease
Structure domains: Restriction Endonuclease
DNA (5'-D(*AP*GP*TP*CP*CP*AP*CP*CP*GP*GP*GP*GP*GP*AP*CP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
Source organism: Streptomyces griseus
Expression system: Not provided
DNA (5'-D(*AP*GP*TP*CP*CP*CP*CP*CP*GP*GP*TP*GP*GP*AP*CP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.88 KDa
Source organism: Streptomyces griseus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21212
Unit cell:
a: 108.682Å b: 133.662Å c: 64.748Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.248
Expression systems:
  • Escherichia coli
  • Not provided