PDBe 3n0e

X-ray diffraction
2.5Å resolution

Crystal structure of WDR5 mutant (W330Y)

Released:
Source organism: Homo sapiens
Primary publication:
The effect of Asp-His-Ser/Thr-Trp tetrad on the thermostability of WD40-repeat proteins.
Biochemistry 49 10237-45 (2010)
PMID: 20939513

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
WD repeat-containing protein 5 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61964 (Residues: 31-334; Coverage: 91%)
Gene names: BIG3, WDR5
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002+
Spacegroup: P21
Unit cell:
a: 43.748Å b: 79.68Å c: 45.79Å
α: 90° β: 101.91° γ: 90°
R-values:
R R work R free
0.222 0.217 0.323
Expression system: Escherichia coli