› 3mu7

X-ray diffraction
1.29Å resolution

Crystal structure of the xylanase and alpha-amylase inhibitor protein (XAIP-II) from scadoxus multiflorus at 1.2 A resolution

Released:
DOI: 10.2210/pdb3mu7/pdb
PDB entry 3mu7 coloured by chain, front view.
PDB entry 3mu7 coloured by chain, side view.
PDB entry 3mu7 coloured by chain, top view.
Source organism: Scadoxus multiflorus
Primary publication:
Modulation of inhibitory activity of xylanase-α-amylase inhibitor protein (XAIP): binding studies and crystal structure determination of XAIP-II from Scadoxus multiflorus at 1.2 Å resolution.
Kumar S, Singh N, Mishra B, Dube D, Sinha M, Singh SB, Dey S, Kaur P, Sharma S, Singh TP
BMC Struct Biol 10 41 (2010)
PMID: 21092126

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 29.53 KDa
Source organism: Scadoxus multiflorus
UniProt:
  • Canonical: D9MWI4 (Residues: 1-273; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 18
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand PEG 7 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 42.152Å b: 64.398Å c: 48.594Å
α: 90° β: 102.1° γ: 90°
R-values:
R R work R free
0.153 0.153 0.171

Quick links

Citations

2 review citations

Structure, Function and Protein Engineering of Cereal-Type Inhibitors Acting on Amylolytic Enzymes.
Møller et al. (2022)
1 more

2 mentions without citation

Structural investigation of a novel N-acetyl glucosamine binding chi-lectin which reveals evolutionary relationship with class III chitinases.
Patil et al. (2013)
1 more