3mm4

X-ray diffraction
2Å resolution

Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana

Released:

Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine kinase CKI1 Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 23.23 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O22267 (Residues: 944-1122; Coverage: 16%)
Gene names: At2g47430, CKI1, T30B22.27
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2221
Unit cell:
a: 54.458Å b: 99.825Å c: 79.943Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.229
Expression system: Escherichia coli BL21(DE3)