PDBe 3mks

X-ray diffraction
2.6Å resolution

Crystal Structure of yeast Cdc4/Skp1 in complex with an allosteric inhibitor SCF-I2

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell division control protein 4 Chains: B, D
Molecule details ›
Chains: B, D
Length: 464 amino acids
Theoretical weight: 52.86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07834 (Residues: 263-744; Coverage: 59%)
Gene names: CDC4, YFL009W
Sequence domains:
Structure domains:
Suppressor of kinetochore protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 169 amino acids
Theoretical weight: 19.5 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52286 (Residues: 2-194; Coverage: 85%)
Gene names: CBF3D, D9798.14, SKP1, YDR328C
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P32
Unit cell:
a: 108.281Å b: 108.281Å c: 165.594Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.211 0.266
Expression system: Escherichia coli BL21(DE3)