PDBe 3mkq

X-ray diffraction
2.5Å resolution

Crystal structure of yeast alpha/betaprime-COP subcomplex of the COPI vesicular coat

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Coatomer subunit beta' Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 814 amino acids
Theoretical weight: 91.4 KDa
Source organism: Saccharomyces cerevisiae YJM789
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A6ZU46 (Residues: 1-814; Coverage: 92%)
Gene names: SCY_1929, SEC27
Sequence domains:
Structure domains:
Coatomer subunit alpha Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 177 amino acids
Theoretical weight: 19.74 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53622 (Residues: 642-818; Coverage: 15%)
Gene names: COP1, D1578, RET1, SEC33, SOO1, YDL145C
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 152.638Å b: 152.638Å c: 294.535Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.272
Expression system: Spodoptera frugiperda