PDBe 3mk3

X-ray diffraction
3.57Å resolution

Crystal structure of Lumazine synthase from Salmonella typhimurium LT2

Released:

Function and Biology Details

Reaction catalysed:
1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6- (D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H(2)O + phosphate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo 60-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6,7-dimethyl-8-ribityllumazine synthase Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x, y
Molecule details ›
Chains: 1, 2, 3, 4, 5, 6, 7, 8, 9, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x, y
Length: 156 amino acids
Theoretical weight: 16.02 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P66038 (Residues: 1-156; Coverage: 100%)
Gene names: STM0417, ribH
Sequence domains: 6,7-dimethyl-8-ribityllumazine synthase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 154.234Å b: 151.503Å c: 235.032Å
α: 90° β: 97.08° γ: 90°
R-values:
R R work R free
0.226 0.226 0.264
Expression system: Escherichia coli