PDBe 3mi9

X-ray diffraction
2.1Å resolution

Crystal structure of HIV-1 Tat complexed with human P-TEFb

Released:

Function and Biology Details

Reactions catalysed:
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. 
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-T1 Chain: B
Molecule details ›
Chain: B
Length: 266 amino acids
Theoretical weight: 30.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O60563 (Residues: 1-266; Coverage: 37%)
Gene name: CCNT1
Sequence domains: Cyclin, N-terminal domain
Structure domains: Cyclin A; domain 1
Protein Tat Chain: C
Molecule details ›
Chain: C
Length: 86 amino acids
Theoretical weight: 9.85 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04608 (Residues: 1-86; Coverage: 100%)
  • Best match: P04608-2 (Residues: 1-72)
Gene name: tat
Sequence domains: Transactivating regulatory protein (Tat)
Cyclin-dependent kinase 9 Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P50750 (Residues: 1-345; Coverage: 93%)
Gene names: CDC2L4, CDK9, TAK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 132.306Å b: 132.306Å c: 95.358Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.264
Expression system: Spodoptera frugiperda