PDBe 3m2t

X-ray diffraction
2.3Å resolution

The crystal structure of dehydrogenase from Chromobacterium violaceum

Released:
Source organism: Chromobacterium violaceum
Entry authors: Zhang Z, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 40.57 KDa
Source organism: Chromobacterium violaceum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7NY68 (Residues: 2-349; Coverage: 99%)
Gene name: CV_1407
Sequence domains: Oxidoreductase family, NAD-binding Rossmann fold
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 138.86Å b: 63.386Å c: 103.629Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.23
Expression system: Escherichia coli BL21(DE3)