› 3m2e

X-ray diffraction
1.4Å resolution

Crystallographic and Single Crystal Spectral Analysis of the Peroxidase Ferryl Intermediate

Released:
DOI: 10.2210/pdb3m2e/pdb
PDB entry 3m2e coloured by chain, front view.
PDB entry 3m2e coloured by chain, side view.
PDB entry 3m2e coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystallographic and single-crystal spectral analysis of the peroxidase ferryl intermediate.
Meharenna YT, Doukov T, Li H, Soltis SM, Poulos TL
Biochemistry 49 2984-6 (2010)
PMID: 20230048

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c peroxidase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00431 (Residues: 71-361; Coverage: 81%)
Gene names: CCP, CCP1, CPO, YKR066C
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 107.709Å b: 75.146Å c: 51.493Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 0.117 not available
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
9 more

12 mentions without citation

Protein Supramolecular Structures: From Self-Assembly to Nanovaccine Design.
Zottig et al. (2020)
11 more