› 3m07

X-ray diffraction
1.4Å resolution

1.4 Angstrom Resolution Crystal Structure of Putative alpha Amylase from Salmonella typhimurium.

Released:
DOI: 10.2210/pdb3m07/pdb
PDB entry 3m07 coloured by chain, front view.
PDB entry 3m07 coloured by chain, side view.
PDB entry 3m07 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Winsor J, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malto-oligosyltrehalose trehalohydrolase Chain: A
Molecule details ›
Chain: A
Length: 618 amino acids
Theoretical weight: 68.59 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZPF0 (Residues: 1-594; Coverage: 100%)
Gene name: STM1560
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 6 x MG
Ligand CL 4 x CL
Ligand BTB 1 x BTB
Ligand PG4 2 x PG4
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 174.048Å b: 51.534Å c: 57.014Å
α: 90° β: 101.18° γ: 90°
R-values:
R R work R free
0.149 0.148 0.173
Expression system: Escherichia coli BL21(DE3)

Quick links