› 3ly4

X-ray diffraction
1.8Å resolution

Crystal Structure of fluorophore-labeled Class A -lactamase PenP-E166Cb in complex with penicillin G

Released:
DOI: 10.2210/pdb3ly4/pdb
PDB entry 3ly4 coloured by chain, front view.
PDB entry 3ly4 coloured by chain, side view.
PDB entry 3ly4 coloured by chain, top view.
Source organism: Bacillus licheniformis
Entry authors: Zhao YX, Leung YC, Wong WT

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133594 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.59 KDa
Source organism: Bacillus licheniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P00808 (Residues: 47-303; Coverage: 92%)
Gene names: blaP, penP
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand PNM 1 x PNM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 43.454Å b: 74.7Å c: 78.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.26
Expression system: Escherichia coli

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