3lwr

X-ray diffraction
2.2Å resolution

Structure of H/ACA RNP bound to a substrate RNA containing 4SU

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-181668 (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (5 distinct):
Probable tRNA pseudouridine synthase B Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.63 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7LWY0 (Residues: 1-340; Coverage: 100%)
Gene names: PF1785, truB
Sequence domains:
Structure domains:
Ribosome biogenesis protein Nop10 Chain: B
Molecule details ›
Chain: B
Length: 60 amino acids
Theoretical weight: 7.24 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1R4 (Residues: 1-60; Coverage: 100%)
Gene names: PF1141, nop10
Sequence domains: Nucleolar RNA-binding protein, Nop10p family
Structure domains: H/ACA ribonucleoprotein complex, subunit Nop10
Large ribosomal subunit protein eL8 Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 13.41 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U160 (Residues: 1-123; Coverage: 100%)
Gene names: PF1367, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
H/ACA RNA Chain: D
Molecule details ›
Chain: D
Length: 58 nucleotides
Theoretical weight: 18.69 KDa
Sequence domains: Pab160 RNA
5'-R(*GP*AP*GP*CP*GP*(4SU)P*GP*CP*GP*GP*UP*UP*U)-3' Chain: E
Molecule details ›
Chain: E
Length: 13 nucleotides
Theoretical weight: 4.21 KDa

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 187.355Å b: 64.434Å c: 83.388Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.222 0.259
Expression system: Escherichia coli