PDBe 3lvw

X-ray diffraction
2.5Å resolution

Glutathione-inhibited ScGCL


Function and Biology Details

Reaction catalysed:
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Glutamate--cysteine ligase Chain: A
Molecule details ›
Chain: A
Length: 692 amino acids
Theoretical weight: 79.93 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
  • Canonical: P32477 (Residues: 1-678; Coverage: 100%)
Gene names: GSH1, J0832, YJL101C
Sequence domains: Glutamate-cysteine ligase
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43212
Unit cell:
a: 118.127Å b: 118.127Å c: 165.799Å
α: 90° β: 90° γ: 90°
R R work R free
0.202 0.199 0.251
Expression system: Escherichia coli