› 3luf

X-ray diffraction
1.76Å resolution

Structure of probable two-component system response regulator/GGDEF domain protein

Released:
DOI: 10.2210/pdb3luf/pdb
PDB entry 3luf coloured by chain, front view.
PDB entry 3luf coloured by chain, side view.
PDB entry 3luf coloured by chain, top view.
Source organism: Aeromonas salmonicida subsp. salmonicida A449
Entry authors: Ramagopal UA, Toro R, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 29.86 KDa
Source organism: Aeromonas salmonicida subsp. salmonicida A449
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4SNL2 (Residues: 2-249; Coverage: 60%)
Gene name: ASA_2441
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand DMS 3 x DMS
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 41.195Å b: 53.699Å c: 57.887Å
α: 75.39° β: 80.99° γ: 77.41°
R-values:
R R work R free
0.189 0.187 0.226
Expression system: Escherichia coli BL21(DE3)

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