PDBe 3lt5

X-ray diffraction
2.3Å resolution

X-ray Crystallographic structure of a Pseudomonas Aeruginosa Azoreductase in complex with balsalazide

Released:
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
A novel mechanism for azoreduction.
J. Mol. Biol. 400 24-37 (2010)
PMID: 20417637

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FMN-dependent NADH-azoreductase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.08 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW Q9I5F3 (Residues: 1-212; Coverage: 100%)
Gene names: PA0785, azoR1
Sequence domains: Flavodoxin-like fold
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3121
Unit cell:
a: 83.097Å b: 83.097Å c: 109.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.154 0.222
Expression system: Escherichia coli BL21(DE3)