› 3ljp

X-ray diffraction
2.2Å resolution

Crystal structure of choline oxidase V464A mutant

Released:
DOI: 10.2210/pdb3ljp/pdb
PDB entry 3ljp coloured by chain, front view.
PDB entry 3ljp coloured by chain, side view.
PDB entry 3ljp coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Role of valine 464 in the flavin oxidation reaction catalyzed by choline oxidase.
Finnegan S, Agniswamy J, Weber IT, Gadda G
Biochemistry 49 2952-61 (2010)
PMID: 20184377

Function and Biology Details

Reaction catalysed: 
(1a) choline + O(2) = betaine aldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 546 amino acids
Theoretical weight: 59.87 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7X2H8 (Residues: 1-546; Coverage: 100%)
Gene name: codA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FDA 2 x FDA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 87.04Å b: 87.04Å c: 353.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.165 0.227
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Alteration of Electron Acceptor Preferences in the Oxidative Half-Reaction of Flavin-Dependent Oxidases and Dehydrogenases.
Hiraka et al. (2020)
11 other articles

3 mentions without citation

Human choline dehydrogenase: medical promises and biochemical challenges.
Salvi et al. (2013)
2 more