PDBe 3les

X-ray diffraction
2.77Å resolution

2F5 Epitope scaffold ES2

Released:
Source organism: Thermus aquaticus
Primary publication:
Elicitation of structure-specific antibodies by epitope scaffolds.
Proc. Natl. Acad. Sci. U.S.A. 107 17880-7 (2010)
PMID: 20876137

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase sigma factor SigA Chains: A, B
Molecule details ›
Chains: A, B
Length: 179 amino acids
Theoretical weight: 20.21 KDa
Source organism: Thermus aquaticus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9EZJ8 (Residues: 93-271; Coverage: 41%)
Gene name: sigA
Sequence domains: Sigma-70 factor, region 1.2

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 37.257Å b: 49.987Å c: 57.567Å
α: 78.03° β: 89.83° γ: 87.57°
R-values:
R R work R free
0.23 0.227 0.26
Expression system: Homo sapiens