PDBe 3l9j

X-ray diffraction
2.1Å resolution

Selection of a novel highly specific TNFalpha antagonist: Insight from the crystal structure of the antagonist-TNFalpha complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tetranectin Chain: C
Molecule details ›
Chain: C
Length: 134 amino acids
Theoretical weight: 15.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05452 (Residues: 67-201; Coverage: 74%)
Gene names: CLEC3B, TNA
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
Tumor necrosis factor, soluble form Chain: T
Molecule details ›
Chain: T
Length: 149 amino acids
Theoretical weight: 16.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01375 (Residues: 85-233; Coverage: 64%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12, MAX II BEAMLINE I911-3
Spacegroup: P6322
Unit cell:
a: 83.905Å b: 83.905Å c: 149.172Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.217
Expression system: Escherichia coli