› 3l31

X-ray diffraction
2.3Å resolution

Crystal structure of the CBS and DRTGG domains of the regulatory region of Clostridium perfringens pyrophosphatase complexed with the inhibitor, AMP

Released:
DOI: 10.2210/pdb3l31/pdb
PDB entry 3l31 coloured by chain, front view.
PDB entry 3l31 coloured by chain, side view.
PDB entry 3l31 coloured by chain, top view.
Source organism: Clostridium perfringens str. 13
Primary publication:
Crystal structures of the CBS and DRTGG domains of the regulatory region of Clostridiumperfringens pyrophosphatase complexed with the inhibitor, AMP, and activator, diadenosine tetraphosphate.
Tuominen H, Salminen A, Oksanen E, Jämsen J, Heikkilä O, Lehtiö L, Magretova NN, Goldman A, Baykov AA, Lahti R
J Mol Biol 398 400-13 (2010)
PMID: 20303981

Function and Biology Details

Reaction catalysed: 
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cobalt-dependent inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.04 KDa
Source organism: Clostridium perfringens str. 13
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8XIQ9 (Residues: 66-306; Coverage: 44%)
Gene name: CPE2055
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 58.96Å b: 79.61Å c: 116.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.277
Expression system: Escherichia coli

Quick links

Citations

4 review citations

CBS domains: Ligand binding sites and conformational variability.
Ereño-Orbea et al. (2013)
3 more

2 mentions without citation

Structural basis of regulation and oligomerization of human cystathionine β-synthase, the central enzyme of transsulfuration.
Ereño-Orbea et al. (2013)
1 more