› 3l0c

X-ray diffraction
2.45Å resolution

Crystal structure of SCP1 phosphatase D206A mutant with trapped inorganic phosphate

Released:
DOI: 10.2210/pdb3l0c/pdb
PDB entry 3l0c coloured by chain, front view.
PDB entry 3l0c coloured by chain, side view.
PDB entry 3l0c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of the phosphoryl transfer reaction mediated by the human small C-terminal domain phosphatase, Scp1.
Zhang M, Liu J, Kim Y, Dixon JE, Pfaff SL, Gill GN, Noel JP, Zhang Y
Protein Sci 19 974-86 (2010)
PMID: 20222012

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190456 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZU7 (Residues: 77-256; Coverage: 69%)
Gene names: CTDSP1, NIF3, NLIIF, SCP1
Sequence domains: NLI interacting factor-like phosphatase
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 125.508Å b: 77.256Å c: 63.116Å
α: 90° β: 112.52° γ: 90°
R-values:
R R work R free
0.207 0.205 0.254
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Human HAD phosphatases: structure, mechanism, and roles in health and disease.
Seifried et al. (2013)
7 more

3 mentions without citation

Targeting the C-Terminal Domain Small Phosphatase 1.
Rallabandi et al. (2020)
2 more