PDBe 3kse

X-ray diffraction
1.71Å resolution

Unreduced cathepsin L in complex with stefin A

Released:
Source organism: Homo sapiens
Entry authors: Renko M, Turk D

Function and Biology Details

Reaction catalysed:
Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cystatin-A Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 98 amino acids
Theoretical weight: 11.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01040 (Residues: 1-98; Coverage: 100%)
Gene names: CSTA, STF1, STFA
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,
Cathepsin L1 heavy chain Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 220 amino acids
Theoretical weight: 24.21 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P07711 (Residues: 114-333; Coverage: 70%)
Gene names: CTSL, CTSL1
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P1
Unit cell:
a: 35.233Å b: 83.948Å c: 83.906Å
α: 118.07° β: 98.04° γ: 98.04°
R-values:
R R work R free
0.16 0.153 0.204
Expression systems:
  • Escherichia coli BL21(DE3)
  • Komagataella pastoris