PDBe 3kr3

X-ray diffraction
2.2Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
antibody-Fab (heavy chain) Chain: H
Molecule details ›
Chain: H
Length: 251 amino acids
Theoretical weight: 26.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody-Fab (light chain) Chain: L
Molecule details ›
Chain: L
Length: 215 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Insulin-like growth factor II Chain: D
Molecule details ›
Chain: D
Length: 67 amino acids
Theoretical weight: 7.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01344 (Residues: 25-91; Coverage: 43%)
Gene names: IGF2, PP1446
Sequence domains: Insulin/IGF/Relaxin family
Structure domains: Insulin-like, subunit E

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 50.667Å b: 106.921Å c: 110.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.251
Expression system: Escherichia coli