PDBe 3kkv

X-ray diffraction
1.8Å resolution

Structure of PKA with a protein Kinase B-selective inhibitor.

Released:
Source organisms:
Entry authors: Elkins PA, Concha NO

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PKI kinase inhibitor Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Synthetic construct
Expression system: Not provided
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.79 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00517 (Residues: 2-351; Coverage: 100%)
Gene name: PRKACA
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 72.411Å b: 75.996Å c: 80.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.225
Expression systems:
  • Not provided
  • Escherichia coli