PDBe 3ke0

X-ray diffraction
2.7Å resolution

Crystal structure of N370S Glucocerebrosidase at acidic pH.

Released:
Source organism: Homo sapiens
Entry authors: Wei RR, Boucher S, Pan CQ, Edmunds T

Function and Biology Details

Reaction catalysed:
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosylceramidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 497 amino acids
Theoretical weight: 55.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P04062 (Residues: 40-536; Coverage: 100%)
Gene names: GBA, GC, GLUC
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: C2221
Unit cell:
a: 109.465Å b: 285.064Å c: 92.225Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.229
Expression system: Spodoptera frugiperda