› 3kb1

X-ray diffraction
2.9Å resolution

Crystal Structure of the Nucleotide-binding protein AF_226 in complex with ADP from Archaeoglobus fulgidus, Northeast Structural Genomics Consortium Target GR157

Released:
DOI: 10.2210/pdb3kb1/pdb
PDB entry 3kb1 coloured by chain, front view.
PDB entry 3kb1 coloured by chain, side view.
PDB entry 3kb1 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Entry authors: Forouhar F, Lew S, Abashidze M, Seetharaman J, Mao M, Xiao R, Ciccosanti C, Wang H, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-sulfur cluster carrier protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.46 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28015 (Residues: 1-254; Coverage: 100%)
Gene name: AF_2269
Sequence domains: NUBPL iron-transfer P-loop NTPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand ZN 1 x ZN
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 51.44Å b: 67.682Å c: 79.403Å
α: 90° β: 90.09° γ: 90°
R-values:
R R work R free
0.214 0.214 0.265
Expression system: Escherichia coli BL21(DE3)

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