› 3ka4

X-ray diffraction
1.4Å resolution

Frog M-ferritin with cobalt

Released:
DOI: 10.2210/pdb3ka4/pdb
PDB entry 3ka4 coloured by chain, front view.
PDB entry 3ka4 coloured by chain, side view.
PDB entry 3ka4 coloured by chain, top view.
Source organism: Lithobates catesbeianus
Primary publication:
Moving metal ions through ferritin-protein nanocages from three-fold pores to catalytic sites.
Tosha T, Ng HL, Bhattasali O, Alber T, Theil EC
J Am Chem Soc 132 14562-9 (2010)
PMID: 20866049

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin, middle subunit Chain: A
Molecule details ›
Chain: A
Length: 176 amino acids
Theoretical weight: 20.62 KDa
Source organism: Lithobates catesbeianus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07798 (Residues: 1-176; Coverage: 100%)
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

3 bound ligands:
Ligand CO 8 x CO
Ligand CL 8 x CL
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: F432
Unit cell:
a: 183.765Å b: 183.765Å c: 183.765Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.174
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The significance of ferritin in cancer: anti-oxidation, inflammation and tumorigenesis.
Alkhateeb et al. (2013)
8 more

8 mentions without citation

Ferritin: the protein nanocage and iron biomineral in health and in disease.
Theil EC. (2013)
7 more