3jue › Citations

Mechanistic insights into regulated cargo binding by ACAP1 protein.

Bai M, Pang X, Lou J, Zhou Q, Zhang K, Ma J, Li J, Sun F, Hsu VW
J Biol Chem 287 28675-85 (2012)
Related entries: 3t9k, 4f1p

Cited: 18 times
EuropePMC logo PMID: 22645133

Abstract

Coat complexes sort protein cargoes into vesicular transport pathways. An emerging class of coat components has been the GTPase-activating proteins (GAPs) that act on the ADP-ribosylation factor (ARF) family of small GTPases. ACAP1 (ArfGAP with coiled-coil, ankyrin repeat, and PH domains protein 1) is an ARF6 GAP that also acts as a key component of a recently defined clathrin complex for endocytic recycling. Phosphorylation by Akt has been shown to enhance cargo binding by ACAP1 in explaining how integrin recycling is an example of regulated transport. We now shed further mechanistic insights into how this regulation is achieved at the level of cargo binding by ACAP1. We initially defined a critical sequence in the cytoplasmic domain of integrin β1 recognized by ACAP1 and showed that this sequence acts as a recycling sorting signal. We then pursued a combination of structural, modeling, and functional studies, which suggest that phosphorylation of ACAP1 relieves a localized mechanism of autoinhibition in regulating cargo binding. Thus, we have elucidated a key regulatory juncture that controls integrin recycling and also advanced the understanding of how regulated cargo binding can lead to regulated transport.

Articles - 3jue mentioned but not cited (5)

  1. Structural and Energetic Characterization of the Ankyrin Repeat Protein Family. Parra RG, Espada R, Verstraete N, Ferreiro DU. PLoS Comput Biol 11 e1004659 (2015)
  2. Functional analysis of rare variants found in schizophrenia implicates a critical role for GIT1-PAK3 signaling in neuroplasticity. Kim MJ, Biag J, Fass DM, Lewis MC, Zhang Q, Fleishman M, Gangwar SP, Machius M, Fromer M, Purcell SM, McCarroll SA, Rudenko G, Premont RT, Scolnick EM, Haggarty SJ. Mol Psychiatry 22 417-429 (2017)
  3. Mechanistic insights into regulated cargo binding by ACAP1 protein. Bai M, Pang X, Lou J, Zhou Q, Zhang K, Ma J, Li J, Sun F, Hsu VW. J Biol Chem 287 28675-28685 (2012)
  4. The Glo3 GAP crystal structure supports the molecular niche model for ArfGAPs in COPI coats. Xie B, Jung C, Chandra M, Engel A, Kendall AK, Jackson LP. Adv Biol Regul 79 100781 (2021)
  5. CARDIO-PRED: an in silico tool for predicting cardiovascular-disorder associated proteins. Jain P, Thukral N, Gahlot LK, Hasija Y. Syst Synth Biol 9 55-66 (2015)


Reviews citing this publication (3)

  1. To degrade or not to degrade: mechanisms and significance of endocytic recycling. Cullen PJ, Steinberg F. Nat Rev Mol Cell Biol 19 679-696 (2018)
  2. Integrin traffic - the update. De Franceschi N, Hamidi H, Alanko J, Sahgal P, Ivaska J. J Cell Sci 128 839-852 (2015)
  3. Integrins and Cell Metabolism: An Intimate Relationship Impacting Cancer. Ata R, Antonescu CN. Int J Mol Sci 18 E189 (2017)

Articles citing this publication (10)

  1. The Arf6 GTPase-activating proteins ARAP2 and ACAP1 define distinct endosomal compartments that regulate integrin α5β1 traffic. Chen PW, Luo R, Jian X, Randazzo PA. J Biol Chem 289 30237-30248 (2014)
  2. Regulation of Cell Migration and β1 Integrin Trafficking by the Endosomal Adaptor GGA3. Ratcliffe CD, Sahgal P, Parachoniak CA, Ivaska J, Park M. Traffic 17 670-688 (2016)
  3. A PH domain in ACAP1 possesses key features of the BAR domain in promoting membrane curvature. Pang X, Fan J, Zhang Y, Zhang K, Gao B, Ma J, Li J, Deng Y, Zhou Q, Egelman EH, Hsu VW, Sun F. Dev Cell 31 73-86 (2014)
  4. Defining the protein and lipid constituents of tubular recycling endosomes. Farmer T, Xie S, Naslavsky N, Stöckli J, James DE, Caplan S. J Biol Chem 296 100190 (2021)
  5. Mammalian copper homeostasis requires retromer-dependent recycling of the high-affinity copper transporter 1. Curnock R, Cullen PJ. J Cell Sci 133 jcs249201 (2020)
  6. Cellular homologs of the double jelly-roll major capsid proteins clarify the origins of an ancient virus kingdom. Krupovic M, Makarova KS, Koonin EV. Proc Natl Acad Sci U S A 119 e2120620119 (2022)
  7. The protein kinase Akt acts as a coat adaptor in endocytic recycling. Hsu JW, Bai M, Li K, Yang JS, Chu N, Cole PA, Eck MJ, Li J, Hsu VW. Nat Cell Biol 22 927-933 (2020)
  8. Coronin2A links actin-based endosomal processes to the EHD1 fission machinery. Dhawan K, Naslavsky N, Caplan S. Mol Biol Cell 33 ar107 (2022)
  9. ACAP1 assembles into an unusual protein lattice for membrane deformation through multiple stages. Chan C, Pang X, Zhang Y, Niu T, Yang S, Zhao D, Li J, Lu L, Hsu VW, Zhou J, Sun F, Fan J. PLoS Comput Biol 15 e1007081 (2019)
  10. ArfGAPs: key regulators for receptor sorting. Shiba Y, Randazzo PA. Receptors Clin Investig 1 e158 (2014)


Quick links