› 3jqr

X-ray diffraction
2.3Å resolution

Crystal structure of the H286L mutant of Ferredoxin-NADP+ reductase from Plasmodium falciparum

Released:
DOI: 10.2210/pdb3jqr/pdb
PDB entry 3jqr coloured by chain, front view.
PDB entry 3jqr coloured by chain, side view.
PDB entry 3jqr coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Plasmodium falciparum ferredoxin-NADP+ reductase His286 plays a dual role in NADP(H) binding and catalysis.
Crobu D, Canevari G, Milani M, Pandini V, Vanoni MA, Bolognesi M, Zanetti G, Aliverti A
Biochemistry 48 9525-33 (2009)
PMID: 19736991

Function and Biology Details

Reaction catalysed: 
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin--NADP reductase, apicoplast Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 37.29 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: C6KT68 (Residues: 56-371; Coverage: 85%)
Gene names: FNR, PF3D7_0623200
Sequence domains: Oxidoreductase NAD-binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 82.109Å b: 96.689Å c: 46.85Å
α: 90° β: 118° γ: 90°
R-values:
R R work R free
0.23 0.227 0.29
Expression system: Escherichia coli

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Citations

1 review citation

Targeting apicoplasts in malaria parasites.
Goodman et al. (2013)
6 other articles