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Structure of the mammalian ribosomal pre-termination complex associated with eRF1.eRF3.GDPNP.

des Georges A, Hashem Y, Unbehaun A, Grassucci RA, Taylor D, Hellen CU, Pestova TV, Frank J
Nucleic Acids Res 42 3409-18 (2014)
Cited: 47 times
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Abstract

Eukaryotic translation termination results from the complex functional interplay between two release factors, eRF1 and eRF3, in which GTP hydrolysis by eRF3 couples codon recognition with peptidyl-tRNA hydrolysis by eRF1. Here, we present a cryo-electron microscopy structure of pre-termination complexes associated with eRF1•eRF3•GDPNP at 9.7 -Å resolution, which corresponds to the initial pre-GTP hydrolysis stage of factor attachment and stop codon recognition. It reveals the ribosomal positions of eRFs and provides insights into the mechanisms of stop codon recognition and triggering of eRF3's GTPase activity.

Articles - 3j5y mentioned but not cited (5)

  1. Structure of the mammalian ribosomal pre-termination complex associated with eRF1.eRF3.GDPNP. des Georges A, Hashem Y, Unbehaun A, Grassucci RA, Taylor D, Hellen CU, Pestova TV, Frank J. Nucleic Acids Res. 42 3409-3418 (2014)
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  11. Embraced by eIF3: structural and functional insights into the roles of eIF3 across the translation cycle. Valášek LS, Zeman J, Wagner S, Beznosková P, Pavlíková Z, Mohammad MP, Hronová V, Herrmannová A, Hashem Y, Gunišová S. Nucleic Acids Res. 45 10948-10968 (2017)
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  14. Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution. Hilal T, Yamamoto H, Loerke J, Bürger J, Mielke T, Spahn CM. Nat Commun 7 13521 (2016)
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  19. Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA. Powers KT, Stevenson-Jones F, Yadav SKN, Amthor B, Bufton JC, Borucu U, Shen D, Becker JP, Lavysh D, Hentze MW, Kulozik AE, Neu-Yilik G, Schaffitzel C. Nucleic Acids Res 49 7665-7679 (2021)
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  24. Chemical footprinting reveals conformational changes of 18S and 28S rRNAs at different steps of translation termination on the human ribosome. Bulygin KN, Bartuli YS, Malygin AA, Graifer DM, Frolova LY, Karpova GG. RNA 22 278-289 (2016)
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