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Structure of the mammalian ribosomal pre-termination complex associated with eRF1-eRF3-GDPNP

Electron Microscopy
9.7Å resolution
PDB entry 3j5y coloured by chain, front view.
PDB entry 3j5y coloured by chain, side view.
PDB entry 3j5y coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules
2 distinct RNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: ERF1-ERF3 translation release factor complex, GSPT1 variant and RNA
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Multimeric state: hetero tetramer
Accessible surface area: 55248.49 Å2
Buried surface area: 5524.19 Å2
Dissociation area: 850.05 Å2
Dissociation energy (ΔGdiss): -5.38 kcal/mol
Dissociation entropy (TΔSdiss): 24.16 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-147261

Macromolecules

Eukaryotic peptide chain release factor subunit 1
Chain: A
Length: 414 amino acids
Theoretical weight: 46.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62495 (Residues: 7-420; Coverage: 95%)
Gene names: ERF1, ETF1, RF1, SUP45L1
Pfam:
InterPro:
Eukaryotic peptide chain release factor subunit 1 in PDB entry 3j5y, assembly 1, front view.
Eukaryotic peptide chain release factor subunit 1 in PDB entry 3j5y, assembly 1, side view.
Eukaryotic peptide chain release factor subunit 1 in PDB entry 3j5y, assembly 1, top view.
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Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Chain: B
Length: 428 amino acids
Theoretical weight: 48.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P15170 (Residues: 69-496; Coverage: 86%)
Gene names: ERF3A, GSPT1
Pfam:
InterPro:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A in PDB entry 3j5y, assembly 1, front view.
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A in PDB entry 3j5y, assembly 1, side view.
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A in PDB entry 3j5y, assembly 1, top view.
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RNA chain C
Name: 5'-R(*AP*UP*UP*GP*UP*AP*AP*AP*AP*A)-3'
Representative chains: C
Length: 10 nucleotides
Theoretical weight: 3.19 KDa
5'-R(*AP*UP*UP*GP*UP*AP*AP*AP*AP*A)-3' in PDB entry 3j5y, assembly 1, front view.
5'-R(*AP*UP*UP*GP*UP*AP*AP*AP*AP*A)-3' in PDB entry 3j5y, assembly 1, side view.
5'-R(*AP*UP*UP*GP*UP*AP*AP*AP*AP*A)-3' in PDB entry 3j5y, assembly 1, top view.
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RNA chain D
Name: tRNA-Leu
Representative chains: D
Length: 88 nucleotides
Theoretical weight: 28.38 KDa
Rfam: tRNA
tRNA-Leu in PDB entry 3j5y, assembly 1, front view.
tRNA-Leu in PDB entry 3j5y, assembly 1, side view.
tRNA-Leu in PDB entry 3j5y, assembly 1, top view.
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