PDBe 3j5y

Electron Microscopy
9.7Å resolution

Structure of the mammalian ribosomal pre-termination complex associated with eRF1-eRF3-GDPNP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (4 distinct):
Eukaryotic peptide chain release factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 46.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62495 (Residues: 7-420; Coverage: 95%)
Gene names: ERF1, ETF1, RF1, SUP45L1
Sequence domains:
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A Chain: B
Molecule details ›
Chain: B
Length: 428 amino acids
Theoretical weight: 48.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P15170 (Residues: 69-496; Coverage: 86%)
Gene names: ERF3A, GSPT1
Sequence domains:
tRNA-Leu Chain: D
Molecule details ›
Chain: D
Length: 88 nucleotides
Theoretical weight: 28.38 KDa
5'-R(*AP*UP*UP*GP*UP*AP*AP*AP*AP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.19 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.7Å
Relevant EMDB volumes: EMD-5801
Expression system: Escherichia coli BL21