PDBe 3iut

X-ray diffraction
1.2Å resolution

The Crystal Structure of Cruzain in Complex with a Tetrafluorophenoxymethyl Ketone Inhibitor

Released:

Function and Biology Details

Reaction catalysed:
Broad endopeptidase specificity similar to that of cathepsin L. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cruzipain Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 23.5 KDa
Source organism: Trypanosoma cruzi
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P25779 (Residues: 123-337; Coverage: 48%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 44.081Å b: 51.451Å c: 45.853Å
α: 90° β: 115.5° γ: 90°
R-values:
R R work R free
0.128 0.127 0.153
Expression system: Komagataella pastoris