PDBe 3it8

X-ray diffraction
2.8Å resolution

Crystal structure of TNF alpha complexed with a poxvirus MHC-related TNF binding protein

Released:
Primary publication:
Crystal structure of TNFalpha complexed with a poxvirus MHC-related TNF binding protein.
Nat. Struct. Mol. Biol. 16 1189-91 (2009)
PMID: 19838188

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2L protein Chains: D, E, F, J, K, L
Molecule details ›
Chains: D, E, F, J, K, L
Length: 324 amino acids
Theoretical weight: 37.33 KDa
Source organism: Yaba-like disease virus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9DHW0 (Residues: 17-332; Coverage: 94%)
Gene name: 2L
Structure domains: Immunoglobulins
Tumor necrosis factor, soluble form Chains: A, B, C, G, H, I
Molecule details ›
Chains: A, B, C, G, H, I
Length: 161 amino acids
Theoretical weight: 17.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01375 (Residues: 82-233; Coverage: 65%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 101.656Å b: 170.295Å c: 122.002Å
α: 90° β: 92.04° γ: 90°
R-values:
R R work R free
0.236 0.236 0.266
Expression systems:
  • Trichoplusia ni
  • Escherichia coli